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Bone marrow culture (Calgary/South)
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Test Name:
Bone marrow culture (Calgary/South)
Organism(s)/Disease(s):
Blastomyces
Blastomycosis
Brucella species
Brucellosis
Histoplasma
Histoplasmosis
Salmonella Typhi/Paratyphi
Typhoid fever/enteric fever
Body Source(s):
Bone marrow aspirate
Bone marrow biopsy
Clinical Prerequisite(s):
Provision of relevant, clinical information is important to ensure a clinically relevant result.
Always provide/record:

•  clinical history
•  travel history
•  antimicrobial treatment history
•  Is the patient immunocompromised?
     - steroid and other immuno-suppressive therapy
     - post-transplant
     - hematological or other malignancy
     - history of chronic infarctions

Collection Device/Tube Type:
BacT Anaerobic FN Plus vial (orange top)
BacT Pediatric PF Plus vial (yellow top)
Sodium Polyanethol (SPS) tube
Specimen Requirement(s):
Submit all 3 collection containers:  Bone marrow culture includes aerobic, anaerobic, and fungal cultures
Collection Instructions:
  • Check the bottom the vial - if it is yellow, DO NOT USE (the bottle may be contaminated)
  • Check the expiry date (y/m/d) found on the container label.  If expired, DO NOT USE
  • Disinfect the bottle tops with 70% isopropanol and allow to dry for 1 minute
    • DO NOT USE IODINE
  • Health care provider disinfects the bone marrow aspiration site as per AHS Guidelines
  • After collection, gently mix the bottles by inversion, to prevent clotting
  • Ensure both bottles are labelled with all required patient identifiers
    • DO NOT COVER the 2D or other bottle barcodes with the patient-identifier labels

  • Handling and Storage:
    *Store at Room Temperature
    DO NOT refrigerate
    Handling/Storage Comments:
    Immediately transport the specimen to the testing laboratory

    Turnaround time for:
    Negative Bacterial culture:  5 days
         Negative fungal culture: up to 6 weeks

    Turnaround Times
    See Microbiology Information»

    Last Reviewed:
    04 May 2023

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