- Test Name:
- Tissue culture, prosthetic joint (Edmonton/Central/North)
- Body Source(s):
-
Tissue - prosthetic joint
-
Clinical Prerequisite(s):
-
Provision of relevant clinical information is important to ensure a clinically relevant result
- State specimen source - Name of joint
- Include "Prosthetic joint infection" on requisition
- State current antibiotic therapy and start date
- State special culture requests (e.g. mycobacterial, fungal) if indicated
- Prior antibiotic exposure (within last 2 weeks)
Note:
- Use checklist for Intra-operative specimen collection for diagnosis of prosthetic joint infections
- Antibiotic prophylaxis should ideally be withheld until AFTER specimen collection - Requisition/Forms:
-
Each container must have its own requisition
- Collection Device/Tube Type:
-
Sterile 60 mL plastic container (orange-top).
- Comments:
-
- 5 - 6 containers
- One container per tissue
- Preservative-free saline
- Collection Instructions:
-
1.
Sharp dissection is recommended for excision rather than cautery
2.
Collect 5 to 6 tissues, 3-4 mm in size. DO NOT send swabs
3.
Each tissue to be collected from different suspected area – ideally from the intersurface
4.
Use different instruments for each tissue to avoid cross contamination
5.
Place each piece of tissue in its own separate container. Cover with preservative-free saline. Tissue submitted in a dry sterile container are suboptimal and will compromise the recovery of fastidious organisms
6.
Each tissue require a separate requisition
- Handling and Storage:
-
Room temperature
- Handling/Storage Comments:
-
- Submit to Laboratory as soon as possible (<2 hours ideally)
- Ensure container does not leak and lid is not cross threaded
- Rejection Criteria:
-
- Specimen submitted in unsterile container
- Specimen submitted in formalin
- Turnaround Times
- See Microbiology Information»
- Last Reviewed:
- 24 November 2021
