Because pedibag urines are prone to high rates of contamination with perineal flora (up to 50%), a positive culture should be confirmed by a suprapubic aspirate or in/out (straight) catheter specimen.  A negative result rules out UTI (Reference:  Choosing Wisely:  Pediatric Infection Disease Recommendation).

The diagnosis of Urinary Tract Infection (UTI) is CLINICAL, based on symptoms and signs, NOT laboratory testing. 

Urine should be sent for culture only AFTER there is a strong clinical suspicion of UTI, to find the causative bacterium and its antibiotic susceptibilities

The role of urine culture is to guide the selection of antibiotic therapy

Urine MUST be collected BEFORE starting antibiotics

1.  For hospital, continuing care facilities, and home collections by healthcare workers:
 - Refer to BD Vacutainer® Urine Collection Kit video
 - Transfer urine to grey-top tube IMMEDIATELY after collection
 - Submit grey-top tube only.  Dispose of blue transfer device according to your facility's policy

2.  For home collections by parent/caregiver or in community clinics:
 - Collect specimen using appropriate instructions
 - Place in sterile orange-top container
 - Refrigerate specimen if not being delivered directly to the lab after collection
 - Specimen should be delivered to any outpatient laboratory or a Patient Service Center within 2 hours

Collection Information - Female

Collection Information - Male

Urine submitted in GREY-TOP tubes can be stored and transported at room temperature

Urines submitted in ORANGE-TOP sterile containers MUST be refrigerated after collection and transported with an ice pack to prevent falsely high bacterial counts

Delivery to the laboratory should be as soon as possible (or within 2 hours) to expedite the result